Proteomics Facilities

ProPic™ Latest technology for the automatic excision of protein spots from1D and 2D gels. The ProPic™ takes detected spot co-ordinates from the Proteome 1D Analyser and Progenesis image analysis software, respectivley and excises protein spots into 96 well plates suitable for subsequent protein digestion.
PerkinElmer ProXPRESS Advanced proteomic imaging system optimised for sensitive and high resolution detection and quantitation of low abundant proteins. Capable of imaging a range of fluorescent dyes( e.g. Sypro Ruby™, DIGE, SuperBRIGHTc) visible dyes (e.g. Coomassie, Silver), and chemilluminescence. The ability to select excitation and emission filters futureproofs the instrument for any new fluorescent probes.
PerkinElmer MULTIPROBE II The Multiprobe II is a multifuncional robotic liquid handling station which takes the 96 well plates from the ProPic™ and performs enzymatic digestion and spots the digestion products directly onto the mass spectrometer MALDI target plates. These are then analysed on the SABC's Perseptive Biosystems Voyager DE Pro mass spectrometer.
NonLinear Dynamics PROGENESIS SOFTWARE 2D gels are analysed using NLD's Progenesis software to automatically detect spots accurately and robustly with minimal user intervention. Additionally this software analyses protein expression levels and prepares a "spot cutting" list which is used by the ProPic™ to locate the positions of proteins for excision.
BioRad 2 DIMENSIONAL GEL ELECTROPHORESIS/IEF For the first and second dimension separation of proteins we have a broad array of BioRad electrophoresis equipment including the Protean ii multicell system. This permits 12 gels to be run simultaneously for minium gel to gel variation.